Journal: Journal of Virology

Article Title: Resveratrol-Mediated Gamma Interferon Reduction Prevents Airway Inflammation and Airway Hyperresponsiveness in Respiratory Syncytial Virus-Infected Immunocompromised Mice

doi: 10.1128/jvi.05869-11

Figure Lengend Snippet: FIG. 2. Effect of resveratrol on the recruitment of inflammatory cells recoverable from BALF. Cells were isolated by cytospin and stained with DiffQuik. Cell numbers were assessed by use of a hemo- cytometer. Control, PBS control; RSV, PBS-treated RSV-infected mice; RSV RES, resveratrol (RES)-treated RSV-infected mice. Val- ues are expressed as the means SD (n 6/group). (A) Total cells present in the BALF of the respective treatment groups. (B) Differ- ences in cell types between treatment groups. **, P 0.01 versus control and resveratrol-treated mice.

Article Snippet: Briefly, slides were fixed and stained with DiffQuik (Baxter Healthcare Corp., Miami, FL) for leukocyte differential analysis, and the number of monocytes, lymphocytes, neutrophils, and eosinophils in a total of 200 cells was determined per slide.

Techniques: Isolation, Staining, Cytometry, Control, Infection

Journal: Cancers

Article Title: ALDH1A2 Is a Candidate Tumor Suppressor Gene in Ovarian Cancer

doi: 10.3390/cancers11101553

Figure Lengend Snippet: Effects of forced expression of ALDH1A2 on invasive potential of ovarian cancer cells. ( A , B ) Invasion assay; RMG-I cells transfected with either the empty vector or ALDH1A2 expression vector for 24 h were loaded into the upper chamber and then fetal bovine serum (FBS, 5%) was added to the lower chamber. After 24 h, the cells that invaded the lower chamber were stained with DiffQuik ( A ) and quantified ( B ) under a microscope. Data are expressed as means ± SD. Statistical significance was assessed using an unpaired t -test. *** p < 0.005. ( C ) Sprouting assay; RMG1 cells ectopically expressing ALDH1A2 were embedded with Matrigel and incubated for 24 h. Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm. ( D ) Sprouting assay using RMG1 cells incubated in the presence or absence of ATRA (1 μM, 5 μM). Phase contrast images were observed (left panel). Calcein-AM-stained cells were visualized using a fluorescence microscope (right panel). Original magnification: 4×. Scale bar, 500 μm.

Article Snippet: After 24 h, the membranes were fixed with 100% methanol and stained with a DiffQuik stain kit (Triangle Biomedical Sciences, Inc., Durham, NC, USA).

Techniques: Expressing, Invasion Assay, Transfection, Plasmid Preparation, Staining, Microscopy, Incubation, Fluorescence